Description
CD33, also known as Siglec-3, is a 67 kD glycosylated transmembrane receptor found on myeloid-specific cells. Historically, CD33 was exclusively utilized for flow cytometry. A CD33 for paraffin-embedded tissues has recently been developed and utilized to profile acute myelogenous leukemias. In instances of acute leukemia, the CD33 antibody demonstrated comparable outcomes by immunohistochemical analysis relative to flow cytometric analysis. The CD33 antibody was identified as a valuable marker in the evaluation of myeloid sarcomas. In standard bone marrow trephine biopsies, clone PWS44 detects myeloid and myelomonocytic hematopoiesis as well as mature macrophages; erythroid and megakaryocyte series cells are negative for CD33. In summary, the CD33 antibody may serve as a valuable marker within an antibody panel for the detection of acute leukemias, myeloid proliferative diseases, and myeloid sarcomas in paraffin-embedded tissue specimens.
Specifications
| Intended Use | RUO |
|---|---|
| Format | Concentrate, Predilute |
| Volume | 0.1 ml, 1.0 ml, 20 ml, 6.0 ml |
| Source | Mouse Monoclonal |
| Clone | PWS44 |
| Isotype | IgG2b |
| Antigen | Prokaryotic recombinant protein corresponding to a region of the C2 domain on human CD33 |
| Localization | Cell membrane / cytoplasm |
| Positive Control | Myeloid leukemia |
