pHH3 (RM)

Phosphohistone H3 (pHH3) is a newly identified marker exclusive to mitotic cells. Phosphorylation of Serine 10 on Histone H3 occurs during mitotic chromatin condensation in the late G2 and M phases of the cell cycle, enabling pHH3 to differentiate between mitotic and apoptotic nuclei. The microscopic examination of mitotic patterns using hematoxylin and eosin (H&E) staining is a standard practice in evaluating the prognostic grade of malignancies. The immunohistochemistry (IHC) staining of pHH3 (Ser10) has been found to be analogous to mitotic figure staining in the H&E section. Nonetheless, in certain instances, H&E staining may erroneously categorize mitotic cells as apoptotic bodies or pyknotic nuclei, leading to an underestimation of the mitotic index (MI). Immunohistochemistry with pHH3 may yield a more precise evaluation of all mitotic cells, including those in which Histone H3 has been phosphorylated just before prophase onset. The prognostic importance of the mitotic index utilizing an anti-pHH3 antibody has been demonstrated to be highly valuable in breast cancer, melanoma, and meningiomas. A rabbit monoclonal antibody (RM) specific for phosphorylated Serine 10 of pHH3, designated clone [BC37], has been produced and described using Western blot, ELISA, and immunohistochemistry (IHC). In tonsil and melanoma, pHH3 (RM) exhibits greater staining intensity in mitotic figures than the polyclonal pHH3. Furthermore, pHH3 (RM) does not display granular staining in interphase nuclei, in contrast to the polyclonal pHH3. pHH3 (RM) may provide additional benefits typical of rabbit monoclonal antibodies, such as a particular epitope and constancy across different lots.

Specifications

Intended Use	RUO
Format	Concentrate, Predilute
Volume	0.1 ml, 1.0 ml, 6.0 ml
Source	Rabbit Monoclonal
Clone	BC37, Biocare Clone
Isotype	IgG
Antigen	PhosphoSer10 of Histone H3 protein
Localization	Nuclear
Positive Control	Tonsil or Melanoma
Species Reactivity	Human, Others Not Tested